ただ今実施中のプロモーション | 詳細はこちら >>
47767
Tricarboxylic Acid Cycle Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit

Tricarboxylic Acid Cycle Antibody Sampler Kit #47767

Reviews ()
Citations (0)
Tricarboxylic Acid Cycle Antibody Sampler Kit: Image 1

Western blot analysis of extracts from 293 cells, untransfected (-) or transfected with a Myc-tagged cDNA expression construct encoding human fumarase (hFumarase-Myc; +), using Fumarase (D9C5) Rabbit mAb (upper) and Myc-Tag (71D10) Rabbit mAb #2278 (lower).

Tricarboxylic Acid Cycle Antibody Sampler Kit: Image 2

Immunohistochemical analysis of paraffin-embedded human ductal breast carcinoma using IDH2 (D8E3B) Rabbit mAb.

Tricarboxylic Acid Cycle Antibody Sampler Kit: Image 3

Confocal immunofluorescent analysis of HeLa cells using Citrate Synthase (D7V8B) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Tricarboxylic Acid Cycle Antibody Sampler Kit: Image 4

Confocal immunofluorescent analysis of HeLa cells using ACO2 (D6D9) XP® Rabbit mAb (green), showing colocalization with mitochondria that were labeled with MitoTracker® Red CMXRos (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Tricarboxylic Acid Cycle Antibody Sampler Kit: Image 5

Western blot analysis of extracts from various cell lines using MPC2 (D4I7G) Rabbit mAb.

Tricarboxylic Acid Cycle Antibody Sampler Kit: Image 6

Immunoprecipitation of MPC1 from Huh7 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or MPC1 (D2L9I) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using MPC1 (D2L9I) Rabbit mAb.

Tricarboxylic Acid Cycle Antibody Sampler Kit: Image 7

Confocal immunofluorescent analysis of HeLa cells using DLST (D22B1) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Tricarboxylic Acid Cycle Antibody Sampler Kit: Image 8

Confocal immunofluorescent analysis of HeLa cells using SDHA (D6J9M) XP® Rabbit mAb (green) and β-Actin (8H10D10) Mouse mAb #3700 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Tricarboxylic Acid Cycle Antibody Sampler Kit: Image 9

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Tricarboxylic Acid Cycle Antibody Sampler Kit: Image 10

Western blot analysis of extracts from HepG2 and NIH/3T3 cells using IDH1 Antibody.

Tricarboxylic Acid Cycle Antibody Sampler Kit: Image 11

Western blot analysis of extracts from various cell lines using Fumarase (D9C5) Rabbit mAb.

Tricarboxylic Acid Cycle Antibody Sampler Kit: Image 12

Immunohistochemical analysis of paraffin-embedded K-562 cell pellet (left, high-expressing) or SR cell pellet (right, low-expressing) using IDH2 (D8E3B) Rabbit mAb.

Tricarboxylic Acid Cycle Antibody Sampler Kit: Image 13

Western blot analysis of extracts from various cell lines using Citrate Synthase (D7V8B) Rabbit mAb.

Tricarboxylic Acid Cycle Antibody Sampler Kit: Image 14

Western blot analysis of extracts from various cell lines using ACO2 (D6D9) XP® Rabbit mAb.

Tricarboxylic Acid Cycle Antibody Sampler Kit: Image 15

Western blot analysis of extracts from various cell lines using MPC1 (D2L9I) Rabbit mAb.

Tricarboxylic Acid Cycle Antibody Sampler Kit: Image 16

Immunoprecipitation of DLST from HeLa cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or DLST (D22B1) XP® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using DLST (D22B1) XP® Rabbit mAb. Mouse Anti-rabbit IgG (Light-Chain Specific) (L57A3) mAb #3677 and Anti-mouse IgG, HRP-linked Antibody #7076 were used as secondary antibodies.

Tricarboxylic Acid Cycle Antibody Sampler Kit: Image 17

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using SDHA (D6J9M) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

Tricarboxylic Acid Cycle Antibody Sampler Kit: Image 18

Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using IDH2 (D8E3B) Rabbit mAb.

Tricarboxylic Acid Cycle Antibody Sampler Kit: Image 19

Western blot analysis of extracts from 293 cells, mock transfected (-) or transfected with a construct expressing DDK-tagged full-length human MPC1 protein (hMPC1-DDK; +) or HA-tagged full-length human MPC2 protein (hMPC2-HA; +), using MPC1 (D2L9I) Rabbit mAb (upper), DYKDDDDK Tag (9A3) Mouse mAb #8146 (upper middle), HA-Tag (C29F4) Rabbit mAb #3724 (lower middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower). Both expression vectors were provided by Dr. Jared Rutter's laboratory at the University of Utah School of Medicine.

Tricarboxylic Acid Cycle Antibody Sampler Kit: Image 20

Western blot analysis of extracts from various cell lines using DLST (D22B1) XP® Rabbit mAb.

Tricarboxylic Acid Cycle Antibody Sampler Kit: Image 21

Immunoprecipitation of SDHA from HeLa cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or SDHA (D6J9M) XP® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using SDHA (D6J9M) XP® Rabbit mAb.

Tricarboxylic Acid Cycle Antibody Sampler Kit: Image 22

Immunohistochemical analysis of paraffin-embedded human adenoid cystic carcinoma of the trachea using IDH2 (D8E3B) Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).

Tricarboxylic Acid Cycle Antibody Sampler Kit: Image 23

Western blot analysis of extracts from various cell lines using SDHA (D6J9M) XP® Rabbit mAb.

Tricarboxylic Acid Cycle Antibody Sampler Kit: Image 24

Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using IDH2 (D8E3B) Rabbit mAb.

Tricarboxylic Acid Cycle Antibody Sampler Kit: Image 25

Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using IDH2 (D8E3B) Rabbit mAb.

Tricarboxylic Acid Cycle Antibody Sampler Kit: Image 26

Immunohistochemical analysis of paraffin-embedded human prostate adenocarcinoma using IDH2 (D8E3B) Rabbit mAb.

Tricarboxylic Acid Cycle Antibody Sampler Kit: Image 27

Immunoprecipitation of IDH2 from Hep G2 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is IDH2 (D8E3B) Rabbit mAb. Western blot analysis was performed using IDH2 (D8E3B) Rabbit mAb.

Tricarboxylic Acid Cycle Antibody Sampler Kit: Image 28

Western blot analysis of extracts from MCF7 and ACHN cells using IDH2 (D8E3B) Rabbit mAb.

To Purchase # 47767T
Product # Size Price Inventory
47767T
1 Kit  (9 x 20 µl)

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Fumarase (D9C5) Rabbit mAb 4567 20 µl
  • WB
Hm H Mk M R 49 Rabbit IgG
IDH2 (D8E3B) Rabbit mAb 56439 20 µl
  • WB
  • IP
  • IHC
H M R 43 Rabbit IgG
Citrate Synthase (D7V8B) Rabbit mAb 14309 20 µl
  • WB
  • IF
Hm H Mk M R 45 Rabbit IgG
ACO2 (D6D9) XP® Rabbit mAb 6571 20 µl
  • WB
  • IP
  • IF
Hm H Mk M R 85 Rabbit IgG
MPC2 (D4I7G) Rabbit mAb 46141 20 µl
  • WB
  • IP
H Mk M R 14 Rabbit IgG
MPC1 (D2L9I) Rabbit mAb 14462 20 µl
  • WB
  • IP
H Mk M R 12 Rabbit IgG
DLST (D22B1) XP® Rabbit mAb 11954 20 µl
  • WB
  • IP
  • IF
H 50 Rabbit IgG
SDHA (D6J9M) XP® Rabbit mAb  11998 20 µl
  • WB
  • IP
  • IHC
  • IF
Hm H Mk M R 70 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 
IDH1 Antibody 3997 20 µl
  • WB
H M 46 Rabbit 

Product Description

The Tricarboxylic Acid Cycle Sampler Kit provides an economical means of detecting select components involved in tricarboxylic acid cycle. The kit contains enough primary antibodies to perform at least two western blot experiments per antibody.

Specificity / Sensitivity

Each antibody in this kit recognizes endogenous levels of its specific target protein. IDH1 (D2H1) Rabbit mAb does not recognize endogenous IDH2 protein, but does recognize IDH2 when recombinantly overexpressed. IDH2 (D8E3B) Rabbit mAb does not cross-react with IDH1 protein. MPC1 (D2L9I) Rabbit mAb does not cross-react with MPC2 protein. MPC2 (D4I7G) Rabbit mAb does not cross-react with MPC1 protein.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly540 of human ACO2 protein, residues near the carboxy terminus of human citrate synthase protein, residues surrounding Pro188 of human DLST protein, residues surrounding Gly354 of human fumarase protein, residues surrounding Arg222 of human IDH1 protein, residues surrounding Val195 of human IDH2 protein, residues near the carboxy terminus of human MPC1 protein, residues surrounding Asn33 of human MPC2 protein and residues surrounding Gly166 of human SDHA protein, respectively.

Background

The tricarboxylic acid (TCA) cycle includes various enzymatic reactions that constitute a key part of cellular aerobic respiration. The transport of the glycolytic end product pyruvate into mitochondria and the decarboxylation of pyruvate in the TCA cycle generate energy through oxidative phosphorylation under aerobic conditions (1,2). Two inner mitochondrial membrane proteins, mitochondrial pyruvate carrier 1 (MPC1) and mitochondrial pyruvate carrier 2 (MPC2), form a 150 kDa complex and are essential proteins in the facilitated transport of pyruvate into mitochondria (1,2). Citrate synthase catalyzes the first and rate-limiting reaction of the TCA cycle (3). Mitochondrial aconitase 2 (ACO2) catalyzes the conversion of citrate to isocitrate via cis-aconitate (4). IDH1 and IDH2 are two of the three isocitrate dehydrogenases that catalyze oxidative decarboxylation of isocitrate to α-ketoglutarate (α-KG) (5). IDH1 functions as a tumor suppressor in the cytoplasm and peroxisomes, whereas IDH2 is in mitochondria and is involved in the TCA cycle (5). Mutations in IDH2 have also been identified in malignant gliomas (6). Dihydrolipoamide succinyltransferase (DLST) is a subunit of the α-ketoglutarate dehydrogenase complex, a key enzymatic complex in the TCA cycle (7). Succinate dehydrogenase subunit A (SDHA) is a component of the TCA cycle and the electron transport chain and is involved in the oxidation of succinate (8). Fumarase catalyzes the conversion of fumarate to malate (9). Fumarase deficiency leads to the accumulation of fumarate, an oncometabolite that has been shown to promote epithelial-to-mesenchymal-transition (EMT), a developmental process that has been implicated in oncogenesis (10).

  1. Herzig, S. et al. (2012) Science 337, 93-6.
  2. Bricker, D.K. et al. (2012) Science 337, 96-100.
  3. Lin, C.C. et al. (2012) Sci Rep 2, 785.
  4. Tohyama, S. et al. (2016) Cell Metab 23, 663-74.
  5. Zhao, S. et al. (2009) Science 324, 261-5.
  6. Yan, H. et al. (2009) N Engl J Med 360, 765-73.
  7. Diaz-Muñoz, M.D. et al. (2015) Nat Immunol 16, 415-25.
  8. Renkema, G.H. et al. (2015) Eur J Hum Genet 23, 202-9.
  9. Wang, T. et al. (2017) Nat Cell Biol 19, 833-843.
  10. Sciacovelli, M. et al. (2016) Nature 537, 544-547.

Pathways & Proteins

Explore pathways + proteins related to this product.

使用に関する制限

法的な権限を与えられたCSTの担当者が署名した書面によって別途明示的に合意された場合を除き、 CST、その関連会社または代理店が提供する製品には以下の条件が適用されます。お客様が定める条件でここに定められた条件に含まれるものを超えるもの、 または、ここに定められた条件と異なるものは、法的な権限を与えられたCSTの担当者が別途書面にて受諾した場合を除き、拒絶され、 いかなる効力も効果も有しません。

研究専用 (For Research Use Only) またはこれに類似する表示がされた製品は、 いかなる目的についても FDA または外国もしくは国内のその他の規制機関により承認、認可または許可を受けていません。 お客様は製品を診断もしくは治療目的で使用してはならず、また、製品に表示された内容に違反する方法で使用してはなりません。 CST が販売または使用許諾する製品は、エンドユーザーであるお客様に対し、使途を研究および開発のみに限定して提供されるものです。 診断、予防もしくは治療目的で製品を使用することまたは製品を再販売 (単独であるか他の製品等の一部であるかを問いません) もしくはその他の商業的利用の目的で購入することについては、CST から別途許諾を得る必要があります。 お客様は以下の事項を遵守しなければなりません。(a) CST の製品 (単独であるか他の資材と一緒であるかを問いません) を販売、使用許諾、貸与、寄付もしくはその他の態様で第三者に譲渡したり使用させたりしてはなりません。また、商用の製品を製造するために CST の製品を使用してはなりません。(b) 複製、改変、リバースエンジニアリング、逆コンパイル、 分解または他の方法により製品の構造または技術を解明しようとしてはなりません。また、 CST の製品またはサービスと競合する製品またはサービスを開発する目的で CST の製品を使用してはなりません。(c) CST の製品の商標、商号、ロゴ、特許または著作権に関する通知または表示を除去したり改変したりしてはなりません。(d) CST の製品をCST 製品販売条件(CST’s Product Terms of Sale) および該当する書面のみに従って使用しなければなりません。(e) CST の製品に関連してお客様が使用する第三者の製品またはサービスに関する使用許諾条件、 サービス提供条件またはこれに類する合意事項を遵守しなければなりません。

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
SignalFire is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.
Tween is a registered trademark of ICI Americas, Inc.