ただ今実施中のプロモーション | 詳細はこちら >>
76696
Non-Homologous End Joining (NHEJ) DNA Repair Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit

Non-Homologous End Joining (NHEJ) DNA Repair Antibody Sampler Kit #76696

Reviews ()
Citations (0)
Non-Homologous End Joining (NHEJ) DNA Repair Antibody Sampler Kit: Image 1

Flow cytometric analysis of M059J cells (blue) and M059K cells (green) using DNA-PKcs (E6U3A) Rabbit mAb (solid lines) or a concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

Non-Homologous End Joining (NHEJ) DNA Repair Antibody Sampler Kit: Image 2

Western blot analysis of extracts from M059K (DNA-PKcs positive) and M059J (DNA-PKcs negative) cells, untreated (-), or treated with neocarzinostatin (0.5 µg/ml, 1 hr; +) to induce DNA double strand breaks, using Phospho-DNA-PKcs (Ser2056) (E9J4G) Rabbit mAb (upper), total DNA-PKcs (E6U3A) Rabbit mAb #38168 (middle), or α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower).

Non-Homologous End Joining (NHEJ) DNA Repair Antibody Sampler Kit: Image 3

Western blot analysis of extracts from various cell lines using Ku70 (D10A7) Rabbit mAb.

Non-Homologous End Joining (NHEJ) DNA Repair Antibody Sampler Kit: Image 4

Confocal immunofluorescent analysis of HeLa cells using Ku80 (C48E7) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).

Non-Homologous End Joining (NHEJ) DNA Repair Antibody Sampler Kit: Image 5

Western blot analysis of extracts from various cell lines using DNA Ligase IV (D5N5N) Rabbit mAb.

Non-Homologous End Joining (NHEJ) DNA Repair Antibody Sampler Kit: Image 6

Western blot analysis of extracts of various cell lines using XLF Antibody.

Non-Homologous End Joining (NHEJ) DNA Repair Antibody Sampler Kit: Image 7

Immunoprecipitation of Artemis from HT-29 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Artemis (D7O8V) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using Artemis (D7O8V) Rabbit mAb.

Non-Homologous End Joining (NHEJ) DNA Repair Antibody Sampler Kit: Image 8

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Non-Homologous End Joining (NHEJ) DNA Repair Antibody Sampler Kit: Image 9

Confocal immunofluorescent analysis of M059K cells (left, positive) and M059J cells (right, negative) using DNA-PKcs (E6U3A) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red).

Non-Homologous End Joining (NHEJ) DNA Repair Antibody Sampler Kit: Image 10

Immunohistochemical analysis of frozen SKOV-3 xenograft using Ku80 (C48E7) Rabbit mAb.

Non-Homologous End Joining (NHEJ) DNA Repair Antibody Sampler Kit: Image 11

Western blot analysis of extracts from various cell lines using Artemis (D7O8V) Rabbit mAb.

Non-Homologous End Joining (NHEJ) DNA Repair Antibody Sampler Kit: Image 12

Immunohistochemical analysis of paraffin-embedded human ductal breast carcinoma using DNA-PKcs (E6U3A) Rabbit mAb.

Non-Homologous End Joining (NHEJ) DNA Repair Antibody Sampler Kit: Image 13

Immunohistochemical analysis of paraffin-embedded human glioblastoma using Ku80 (C48E7) Rabbit mAb.

Non-Homologous End Joining (NHEJ) DNA Repair Antibody Sampler Kit: Image 14

Immunohistochemical analysis of paraffin-embedded normal human colon using DNA-PKcs (E6U3A) Rabbit mAb.

Non-Homologous End Joining (NHEJ) DNA Repair Antibody Sampler Kit: Image 15

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Ku80 (C48E7) Rabbit mAb.

Non-Homologous End Joining (NHEJ) DNA Repair Antibody Sampler Kit: Image 16

Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using DNA-PKcs (E6U3A) Rabbit mAb.

Non-Homologous End Joining (NHEJ) DNA Repair Antibody Sampler Kit: Image 17

Immunohistochemical analysis of paraffin-embedded human melanoma using Ku80 (C48E7) Rabbit mAb.

Non-Homologous End Joining (NHEJ) DNA Repair Antibody Sampler Kit: Image 18

Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma using DNA-PKcs (E6U3A) Rabbit mAb.

Non-Homologous End Joining (NHEJ) DNA Repair Antibody Sampler Kit: Image 19

Immunohistochemical analysis of paraffin-embedded human GIST using Ku80 (C48E7) Rabbit mAb.

Non-Homologous End Joining (NHEJ) DNA Repair Antibody Sampler Kit: Image 20

Immunohistochemical analysis of paraffin-embedded human endometriod carcinoma using DNA-PKcs (E6U3A) Rabbit mAb.

Non-Homologous End Joining (NHEJ) DNA Repair Antibody Sampler Kit: Image 21

Western blot analysis of cell extracts from HeLa, A549 and COS cells using Ku80 (C48E7) Rabbit mAb.

Non-Homologous End Joining (NHEJ) DNA Repair Antibody Sampler Kit: Image 22

Immunohistochemical analysis of paraffin-embedded M059K cell pellet (left, positive) or M059J cell pellet (right, negative) using DNA-PKcs (E6U3A) Rabbit mAb.

Non-Homologous End Joining (NHEJ) DNA Repair Antibody Sampler Kit: Image 23

Western blot analysis of extracts from M059K cells, which express normal levels of DNA-PKcs, and M059J cells, which lack DNA-PKcs, using DNA-PKcs (E6U3A) Rabbit mAb (upper) or α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower).

To Purchase # 76696T
Product # Size Price Inventory
76696T
1 Kit  (7 x 20 µl)

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
DNA-PKcs (E6U3A) Rabbit mAb 38168 20 µl
  • WB
  • IHC
  • IF
  • F
H 450 Rabbit IgG
Phospho-DNA-PKcs (Ser2056) (E9J4G) Rabbit mAb 68716 20 µl
  • WB
H 450 Rabbit IgG
Ku70 (D10A7) Rabbit mAb 4588 20 µl
  • WB
H Mk M R 70 Rabbit IgG
Ku80 (C48E7) Rabbit mAb 2180 20 µl
  • WB
  • IP
  • IHC
  • IF
H Mk 86 Rabbit IgG
DNA Ligase IV (D5N5N) Rabbit mAb 14649 20 µl
  • WB
H 100 Rabbit IgG
XLF Antibody 2854 20 µl
  • WB
  • IP
H 39 Rabbit 
Artemis (D7O8V) Rabbit mAb 13381 20 µl
  • WB
  • IP
H Mk 90 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 

Product Description

The Non-Homologous End Joining (NHEJ) DNA Repair Antibody Sampler Kit provides an economical means of detecting proteins involved in NHEJ DNA repair. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.

Specificity / Sensitivity

Each antibody in the Non-Homologous End Joining (NHEJ) DNA Repair Antibody Sampler Kit detects endogenous levels of its target protein. Phospho-DNA-PKcs (Ser2056) (E9J4G) Rabbit mAb recognizes endogenous levels of DNA-PKcs protein only when phosphorylated at Ser2056.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Pro608 of human DNA-PKcs protein, Val294 of mouse Ku70 protein, the carboxy terminus of human Ku80 protein, Leu771 of human DNA ligase IV protein, and Pro367 of human artemis protein. Phosphorylation-specific monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser2056 of human DNA-PKcs protein. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids near the carboxy terminus of human XLF protein. Polyclonal antibodies are purified by peptide affinity chromatography.

Background

DNA double-strand breaks (DSBs) are potentially hazardous lesions that can be induced by ionizing radiation (IR), radiomimetic chemicals, or DNA replication inhibitors. Cells recognize and repair DSBs via two distinct but partly overlapping signaling pathways, non-homologous end joining (NHEJ) and homologous recombination (HR). DNA repair via the HR pathway is restricted to S and G2 phases of the cell cycle, while NHEJ can occur during any phase. NHEJ machinery is also utilized in V(D)J recombination, a process that generates diversity in immunoglobulin and T cell receptor genes. Defects in both pathways have been associated with human disease, including cancer (1).

DNA repair through the NHEJ pathway involves a core group of proteins that includes the Ku heterodimer (Ku70/Ku80), DNA-PKcs, DNA ligase IV, XRCC4, and XLF. XLF interacts with XRCC4 and promotes the ligation of DNA strands by DNA ligase IV and the ligase cofactor XRCC4. The ATP-dependent ligation of free DNA ends is the final step in the NHEJ repair pathway (2). DNA ligase IV and the endonuclease artemis suppress homologous recombination and promote NHEJ (3).

  1. Hartlerode, A.J. and Scully, R. (2009) Biochem J 423, 157-68.
  2. Tsai, C.J. et al. (2007) Proc Natl Acad Sci U S A 104, 7851-6.
  3. Kurosawa, A. et al. (2013) PLoS One 8, e72253.

Pathways & Proteins

Explore pathways + proteins related to this product.

使用に関する制限

法的な権限を与えられたCSTの担当者が署名した書面によって別途明示的に合意された場合を除き、 CST、その関連会社または代理店が提供する製品には以下の条件が適用されます。お客様が定める条件でここに定められた条件に含まれるものを超えるもの、 または、ここに定められた条件と異なるものは、法的な権限を与えられたCSTの担当者が別途書面にて受諾した場合を除き、拒絶され、 いかなる効力も効果も有しません。

研究専用 (For Research Use Only) またはこれに類似する表示がされた製品は、 いかなる目的についても FDA または外国もしくは国内のその他の規制機関により承認、認可または許可を受けていません。 お客様は製品を診断もしくは治療目的で使用してはならず、また、製品に表示された内容に違反する方法で使用してはなりません。 CST が販売または使用許諾する製品は、エンドユーザーであるお客様に対し、使途を研究および開発のみに限定して提供されるものです。 診断、予防もしくは治療目的で製品を使用することまたは製品を再販売 (単独であるか他の製品等の一部であるかを問いません) もしくはその他の商業的利用の目的で購入することについては、CST から別途許諾を得る必要があります。 お客様は以下の事項を遵守しなければなりません。(a) CST の製品 (単独であるか他の資材と一緒であるかを問いません) を販売、使用許諾、貸与、寄付もしくはその他の態様で第三者に譲渡したり使用させたりしてはなりません。また、商用の製品を製造するために CST の製品を使用してはなりません。(b) 複製、改変、リバースエンジニアリング、逆コンパイル、 分解または他の方法により製品の構造または技術を解明しようとしてはなりません。また、 CST の製品またはサービスと競合する製品またはサービスを開発する目的で CST の製品を使用してはなりません。(c) CST の製品の商標、商号、ロゴ、特許または著作権に関する通知または表示を除去したり改変したりしてはなりません。(d) CST の製品をCST 製品販売条件(CST’s Product Terms of Sale) および該当する書面のみに従って使用しなければなりません。(e) CST の製品に関連してお客様が使用する第三者の製品またはサービスに関する使用許諾条件、 サービス提供条件またはこれに類する合意事項を遵守しなければなりません。

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.