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17027
Cancer-associated Growth Factor Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit

Cancer-associated Growth Factor Antibody Sampler Kit #17027

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Cancer-associated Growth Factor Antibody Sampler Kit: Image 1
Western blot analysis of extracts from various cell lines using VEGF-A (E9X8Q) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Negative expression of VEGF-A protein in HuT 102 cells is consistent with the predicted expression pattern.
Cancer-associated Growth Factor Antibody Sampler Kit: Image 2
Western blot analysis of extracts from various cell lines using EREG (D4O5I) Rabbit mAb.
Cancer-associated Growth Factor Antibody Sampler Kit: Image 3
Western blot analysis of extracts from various cell lines using HBEGF (E5L5T) Rabbit mAb (upper) and GAPDH (D16H11) XP®Rabbit mAb #5174 (lower).
Cancer-associated Growth Factor Antibody Sampler Kit: Image 4
Western blot analysis of recombinant active TGF-β1 using TGF-β (56E4) Rabbit mAb.
Cancer-associated Growth Factor Antibody Sampler Kit: Image 5
Immunoprecipitation of VEGF-A protein from U-87 MG cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is VEGF-A (E9X8Q) Rabbit mAb. Western blot analysis was performed using VEGF-A (E9X8Q) Rabbit mAb. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127 was used as a secondary antibody.
Cancer-associated Growth Factor Antibody Sampler Kit: Image 6
Western blot analysis of extracts from various cell lines using Angiopoietin-2 (D200) Antibody (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). The absence of angiopoietin-2 expression in A-431 cell extracts is consistent with molecular and proteomic expression profiling data, confirming specificity of the antibody.
Cancer-associated Growth Factor Antibody Sampler Kit: Image 7
Western blot analysis of recombinant human HGF (rhHGF) and extracts from A-204 and HeLa cells using HGF β (D6S7D) Rabbit mAb (upper) and β-Actin (D6A8) XP® Rabbit mAb #8457 (lower).
Cancer-associated Growth Factor Antibody Sampler Kit: Image 8
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.
Cancer-associated Growth Factor Antibody Sampler Kit: Image 9
Western blot analysis of extracts from SK-N-MC and SUP-HD1 cells, untreated (-) or treated with Brefeldin A (+), using IGF-I (E6B7O) Rabbit mAb (upper) and GAPDH (D16H11) XP®Rabbit mAb #5174 (lower).
Cancer-associated Growth Factor Antibody Sampler Kit: Image 10
Western blot analysis of extracts from various cell lines using MIF (E7T1W) Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). The absence of MIF protein in SNB-19 cells is consistent with reported mRNA expression profiles, confirming specificity of the antibody for MIF.
Cancer-associated Growth Factor Antibody Sampler Kit: Image 11
Western blot analysis of extracts from various cell lines using Basic FGF (E9S5A) Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). The absence of basic FGF protein in Capan-1 cells is consistent with mRNA expression profiles reported in public bioinformatic databases, confirming specificity of the antibody for basic FGF.
Cancer-associated Growth Factor Antibody Sampler Kit: Image 12
Immunoprecipitation of EREG from COLO 205 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or EREG (D4O5I) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using EREG (D4O5I) Rabbit mAb.
Cancer-associated Growth Factor Antibody Sampler Kit: Image 13
Immunoprecipitation of HBEGF/proHBEGF protein from MCAS cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is HBEGF (E5L5T) Rabbit mAb. Western blot analysis was performed using HBEGF (E5L5T) Rabbit mAb. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as a secondary antibody.
Cancer-associated Growth Factor Antibody Sampler Kit: Image 14
Western blot analysis of extracts of HeLa cells, mock transfected or transfected with TGF-β1 precursor, using TGF-β (56E4) Rabbit mAb.
Cancer-associated Growth Factor Antibody Sampler Kit: Image 15
Immunohistochemical analysis of paraffin-embedded human infiltrating ductal carcinoma of the breast HGF β (D6S7D)

XP® Rabbit mAb.

Cancer-associated Growth Factor Antibody Sampler Kit: Image 16
Immunohistochemical analysis of paraffin-embedded human ductal breast carcinoma using MIF (E7T1W) Rabbit mAb.
Cancer-associated Growth Factor Antibody Sampler Kit: Image 17
Immunoprecipitation of basic FGF protein from A-172 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Basic FGF (E9S5A) Rabbit mAb. Western blot analysis was performed using Basic FGF (E9S5A) Rabbit mAb. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as a secondary antibody.
Cancer-associated Growth Factor Antibody Sampler Kit: Image 18
Western blot analysis of extracts from K-562, Saos-2 and 786-0 cells using TGF-β (56E4) Rabbit mAb.
Cancer-associated Growth Factor Antibody Sampler Kit: Image 19
Immunohistochemical analysis of paraffin-embedded A-204 (left) and HeLa (right) cell pellets using HGF β (D6S7D) XP® Rabbit mAb.
Cancer-associated Growth Factor Antibody Sampler Kit: Image 20
Immunohistochemical analysis of paraffin-embedded 293T cell pellet (left, positive) or SNB-19 cell pellet (right, negative) using MIF (E7T1W) Rabbit mAb.
Cancer-associated Growth Factor Antibody Sampler Kit: Image 21
Immunohistochemical analysis of paraffin-embedded human embryonal rhabdomyosarcoma using HGF β (D6S7D) XP® Rabbit mAb.
Cancer-associated Growth Factor Antibody Sampler Kit: Image 22
Immunohistochemical analysis of paraffin-embedded normal mouse kidney using MIF (E7T1W) Rabbit mAb.
Cancer-associated Growth Factor Antibody Sampler Kit: Image 23
Immunohistochemical analysis of paraffin-embedded human uterine rhabdomyosarcoma using HGF β (D6S7D) XP®Rabbit mAb.
Cancer-associated Growth Factor Antibody Sampler Kit: Image 24
Immunohistochemical analysis of paraffin-embedded human ovarian serous carcinoma using MIF (E7T1W) Rabbit mAb.
Cancer-associated Growth Factor Antibody Sampler Kit: Image 25
Immunohistochemical analysis of paraffin-embedded human ovarian clear cell carcinoma using MIF (E7T1W) Rabbit mAb.
Cancer-associated Growth Factor Antibody Sampler Kit: Image 26
Immunohistochemical analysis of paraffin-embedded human prostate adenocarcinoma using MIF (E7T1W) Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).
Cancer-associated Growth Factor Antibody Sampler Kit: Image 27
Immunohistochemical analysis of paraffin-embedded normal human thyroid using MIF (E7T1W) Rabbit mAb.
To Purchase # 17027
Cat. # Size Qty. Price Inventory
17027T
1 Kit

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Basic FGF (E9S5A) Rabbit mAb 98658 20 µl
  • WB
  • IP
H 18, 22, 24 Rabbit IgG
IGF-I (E6B7O) Rabbit mAb 73034 20 µl
  • WB
H M 9, 13, 18, 20 Rabbit IgG
HGF β (D6S7D) XP® Rabbit mAb 52445 20 µl
  • WB
  • IHC
H 35, 85 Rabbit IgG
TGF-β (56E4) Rabbit mAb 3709 20 µl
  • WB
H 12, 45-60 Rabbit 
HBEGF (E5L5T) Rabbit mAb 27450 20 µl
  • WB
  • IP
H 18, 21, 27 Rabbit IgG
MIF (E7T1W) Rabbit mAb 87501 20 µl
  • WB
  • IHC
H M R 12 Rabbit IgG
EREG (D4O5I) Rabbit mAb 12048 20 µl
  • WB
  • IP
H 17,19, 30 Rabbit IgG
Angiopoietin-2 (D200) Antibody 50697 20 µl
  • WB
H 68, 70 Rabbit 
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 
VEGF-A (E9X8Q) Rabbit mAb 50661 20 µl
  • WB
  • IP
H 16, 20, 23, 26 Rabbit IgG

Product Description

The Cancer-associated Growth Factor Antibody Sampler Kit provides an economical means of detecting selected growth factors that have been shown to influence tumor development. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.

Specificity / Sensitivity

Each antibody in the Cancer-associated Growth Factor Antibody Sampler Kit detects endogenous levels of its total target protein. VEGF-A Antibody recognizes endogenous levels of all isoforms of total VEGF-A protein. This antibody also detects a 47 kDa band of unknown identity. TGF-β (56E4) Rabbit mAb detects endogenous levels of TGF-β precursor proteins. The antibody preferentially detects TGF-β1 and TGF-β3 proteins, as determined by western blot analysis of recombinant proteins. HBEGF (E5L5T) Rabbit mAb recognizes endogenous levels of total proHBEGF and mature HBEGF protein. This antibody also detects a 58 kDa band of unknown origin. MIF (E7T1W) Rabbit mAb recognizes endogenous levels of total MIF protein. Non-specific staining by MIF (E7T1W) Rabbit mAb was observed in mouse brain by immunohistochemistry. EREG (D4O5I) Rabbit mAb recognizes endogenous levels of proepiregulin and the C-terminal propeptide of the EREG protein. It does not recognize the mature form of EREG.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with recombinant protein specific to the mature full-length human basic FGF protein, with recombinant protein fragment specific to human mature HBEGF protein, with recombinant protein specific to the carboxy terminus of human HGF protein, with synthetic peptides corresponding to residues surrounding Ser83 of human IGF-I protein, Tyr100 of human MIF protein, and Glu155 of human EREG protein, and with synthetic peptides corresponding to a region in the carboxy terminus of TGF-β1 protein.

Polyclonal antibodies are produced by immunizing animals with a synthetic peptides corresponding to residues surronding His38 of human VEGFA protein and Asp200 of human angiopoietin-2 protein. Antibodies are purified by peptide affinity chromatography.

Background

The tumor microenvironment (TME) is composed of a heterogenous mixture of tumor cells, blood vessels, fibroblasts, stromal cells, infiltrating immune cells, and extracellular matrix (ECM) components, whose collective interactions play important roles in tumor development (1). Cells in the TME secrete a variety of bioactive molecules, including growth factors, cytokines, ECM proteins, and proteases (e.g., MMPs), many of which play critical roles in regulating growth and development of the tumor (2,3). Growth factors play particularly important roles in the TME, serving as cellular messengers that trigger activation or suppression of signaling pathways that govern tumor development, either directly via the tumor cells, or indirectly by way of effects on the TME. Binding of growth factors to their cognate receptors leads to activation of intracellular signaling pathways, resulting in changes in the expression of target genes that regulate cell behavior. Many growth factors (e.g., IGFs, HGFs, FGFs, HBEGF, EREG) are known to promote tumor development by way of direct effects on tumor cells; other growth factors can affect tumor development indirectly, through effects in the TME that influence tumor angiogenesis (e.g., VEGFs, angiopoietins), ECM deposition (TGF-β), or immune cell signaling (e.g., TGF-β, HBEGF, MIF) (4). The diverse and complex role played by growth factors in promoting tumorigenesis makes them important therapeutic targets in oncology, while elucidating the functions of specific growth factors in the context of tumor development remains an active area of cancer research (5).
  1. Quail, D.F. and Joyce, J.A. (2013) Nat Med 19, 1423-37.
  2. Wortzel, I. et al. (2019) Dev Cell 49, 347-360.
  3. Massagué, J. and Obenauf, A.C. (2016) Nature 529, 298-306.
  4. Penticuff, J.C. et al. (2019) Nat Rev Urol 16, 318-328.
  5. Steeg, P.S. (2016) Nat Rev Cancer 16, 201-18.

Pathways & Proteins

Explore pathways + proteins related to this product.

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For Research Use Only. Not For Use In Diagnostic Procedures.
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