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93473
β-Actin (13E5) Rabbit mAb (BSA and Azide Free)
Primary Antibodies
Monoclonal Antibody
R
Recombinant

β-Actin (13E5) Rabbit mAb (BSA and Azide Free) #93473

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  1. WB
  2. IHC
  3. IF
  4. F
Western Blotting Image 1: β-Actin (13E5) Rabbit mAb (BSA and Azide Free)
Western blot analysis of cell extracts from various cell lines using beta-Actin (13E5) Rabbit mAb. Data were generated using the standard formulation of this product.
Western Blotting Image 2: β-Actin (13E5) Rabbit mAb (BSA and Azide Free)
Western blot analysis of recombinant Actin isoforms using β-Actin (13E5) Rabbit mAb (upper) and Pan-Actin Antibody #4968 (lower). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 1: β-Actin (13E5) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human heart using beta-Actin (13E5) Rabbit mAb. Note the lack of staining of cardiac actin. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 2: β-Actin (13E5) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human leiomyoma using beta-Actin (13E5) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 3: β-Actin (13E5) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using beta-Actin (13E5) Rabbit mAb in the presence of control peptide (left) or beta-Actin Blocking Peptide #1025 (right). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 4: β-Actin (13E5) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded 4T1 syngeneic mouse tumor using β-actin (13E5) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 5: β-Actin (13E5) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human skeletal muscle using beta-Actin (13E5) Rabbit mAb. Note the lack of staining of skeletal muscle actin. Data were generated using the standard formulation of this product.
Immunofluorescence Image 1: β-Actin (13E5) Rabbit mAb (BSA and Azide Free)
Confocal immunofluorescent analysis of fixed, frozen mouse pancreas at low magnification (left) and high magnification (right) using β-Actin (13E5) Rabbit mAb #4970 (red) and DAPI #4083 (blue). Data were generated using the standard formulation of this product.
Immunofluorescence Image 1: β-Actin (13E5) Rabbit mAb (BSA and Azide Free)
Confocal immunofluorescent analysis of COS-7 cells using β-Actin (13E5) Rabbit mAb (green). Blue pseudocolor = DRAQ5®#4084 (fluorescent DNA dye). Data were generated using the standard formulation of this product.
Flow Cytometry Image 1: β-Actin (13E5) Rabbit mAb (BSA and Azide Free)
Flow cytometric analysis of HeLa cells using β-Actin (13E5) Rabbit mAb (solid line) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed line). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. Data were generated using the standard formulation of this product.
To Purchase # 93473
Cat. # Size Qty. Price Inventory
93473SF
100 µg

Supporting Data

REACTIVITY H M R Mk B Pg
SENSITIVITY Endogenous
MW (kDa) 45
Source/Isotype Rabbit IgG

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • C&R-CUT&RUN
  • C&T-CUT&Tag
  • DB-Dot Blot
  • eCLIP-eCLIP
  • IF-Immunofluorescence
  • F-Flow Cytometry

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • Rab-Rabbit
  • All-All Species Expected

Product Usage Information

This product is the carrier free version of product #4970. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS, BSA and Azide Free.

For standard formulation of this product see product #4970

Storage

Store at -20°C.This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

β-Actin (13E5) Rabbit mAb (BSA and Azide Free) detects endogenous levels of total β-actin protein. Depsite the high sequence identity between the cytoplasmic actin isoforms, β-actin and cytoplasmic γ-actin, β-Actin (13E5) Rabbit mAb (BSA and Azide Free) #4970 does not cross-react with cytoplasmic γ-actin, or any other actin isoforms.

Species Reactivity:

Human, Mouse, Rat, Monkey, Bovine, Pig

Species predicted to react based on 100% sequence homology:

Hamster, Chicken, Dog, Horse, Rabbit

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino-terminus of human β-actin protein.

Background

Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are ubiquitously expressed, controlling cell structure and motility (1). While all actin isoforms are highly homologous, cytoplasmic β- and γ-actin protein sequences differ by only four biochemically similar amino acids (2). For this reason, antibodies raised to β-actin may cross-react with γ-actin, and vice versa. α-cardiac and α-skeletal actin are expressed in striated cardiac and skeletal muscles, respectively; two smooth muscle actins, α- and γ-actin, are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. These actin isoforms regulate the contractile potential of muscle cells (1). Actin exists mainly as a fibrous polymer, F-actin. In response to cytoskeletal reorganizing signals during processes such as cytokinesis, endocytosis, or stress, cofilin promotes fragmentation and depolymerization of F-actin, resulting in an increase in the monomeric globular form, G-actin (3). The ARP2/3 complex stabilizes F-actin fragments and promotes formation of new actin filaments (3). Research studies have shown that actin is hyperphosphorylated in primary breast tumors (4). Cleavage of actin under apoptotic conditions has been observed in vitro and in cardiac and skeletal muscle, as shown in research studies (5-7). Actin cleavage by caspase-3 may accelerate ubiquitin/proteasome-dependent muscle proteolysis (7).
  1. Herman, I.M. (1993) Curr. Opin. Cell Biol. 5, 48-55.
  2. Perrin, B.J. and Ervasti, J.M. (2010) Cytoskeleton (Hoboken) 67, 630-4.
  3. Condeelis, J. (2001) Trends Cell Biol 11, 288-93.
  4. Lim, Y.P. et al. (2004) Clin Cancer Res 10, 3980-7.
  5. Kayalar, C. et al. (1996) Proc Natl Acad Sci U S A 93, 2234-8.
  6. Communal, C. et al. (2002) Proc Natl Acad Sci U S A 99, 6252-6.
  7. Du, J. et al. (2004) J Clin Invest 113, 115-23.

Pathways & Proteins

Explore pathways + proteins related to this product.

使用に関する制限

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For Research Use Only. Not For Use In Diagnostic Procedures.
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