Western blot analysis of extracts from THP-1 cells differentiated with TPA (12-O-Tetradecanoylphorbol-13-Acetate) #4174 (80 nM, 16 hr) and then untransfected (-) or transfected with 2’,3’-cGAMP (sodium salt) (10 μg/mL, 6 hr; +), using Phospho-STING (Ser366) (D7C3S) Rabbit mAb #19781 (upper) or STING (D2P2F) Rabbit mAb #13647 (lower).
Chemical structure of 2'3'-cGAMP (sodium salt).
2’,3’-cGAMP (sodium salt) is supplied as a lyophilized powder. For a 5 mM stock, reconstitute 500 μg of powder in 139 μl of DMSO. Working concentrations and length of treatment can vary depending on the desired effect.
Store lyophilized at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, continue to store at -20ºC and use within 1 month to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.
Molecular Weight: 718.4 g/mol
Molecular Formula: C20H22N10Na2O13P2
Solubility: Soluble in DMSO at 50 mg/ml and water at 10 mg/ml.
2’,3’-cGAMP (sodium salt), also known as 2’,3’-cyclic-GMP-AMP, is a second messenger produced by the enzyme cGAMP synthase (cGAS) in response to cytoplasmic DNA (1,2). cGAMP binds and activates the stimulator of interferon genes (STING), a transmembrane adaptor protein that is a critical component of the cellular innate immune response to pathogenic cytoplasmic DNA (1-4). The binding affinity of 2’,3’-cGAMP to STING is stronger than other cyclic di-nucleotides such as c-di-GMP, 3′2′-cGAMP, 3′3′-cGAMP, and 2′2′-cGAMP with a reported Kd value of 3.79 nM compared to 1.21 μM, 1.61 μM, 1.04 μM, and 287 nM, respectively (5).
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