PTMScan® Direct PI3K/Akt Service allows access to perform targeted screening and quantification of a defined set of protein sites within the or related to the PI3K/ Akt pathway that are critical for drug discovery, biomarker discovery, or diagnostic development. The table below illustrates the list of protein sites that can be monitored with PTMScan® Direct PI3K/Akt Service.
Altogether, the PTMScan® Direct PI3K / Akt service provides a robust method to qualitatively identify and quantitatively profile 296 unique phosphorylation sites to 105 proteins.
| PI3K / Akt Protein Sites | |||
|---|---|---|---|
| PI3K / Akt Protein Sites | |||
| 4E-BP1 (Ser35) | 4E-BP1 (Ser44) | 4E-BP1 (Thr36) | 4E-BP1 (Thr37) |
| 4E-BP1 (Thr41) | 4E-BP1 (Thr45) | 4E-BP1 (Thr46) | 4E-BP1 (Thr50) |
| 4E-BP1 (Tyr34) | 4E-BP1 (Tyr54) | 4E-BP2 (Ser44) | 4E-BP2 (Thr36) |
| 4E-BP2 (Thr37) | 4E-BP2 (Thr41) | 4E-BP2 (Thr45) | 4E-BP2 (Thr46) |
| 4E-BP2 (Thr50) | 4E-BP2 (Tyr34) | 4E-BP2 (Tyr54) | 4E-BP3 (Thr23) |
| 4E-BP3 (Thr27) | 4E-BP3 (Thr31) | 4E-BP3 (Thr32) | ACTB (Thr201) |
| ACTG1 (Thr201) | Akt1 (Ser473) | Akt1 (Thr305) | Akt1 (Thr308) |
| Akt1 (Thr312) | Akt1 (Tyr315) | Akt2 (Ser474) | Akt2 (Ser476) |
| Akt2 (Ser478) | Akt2 (Thr309) | Akt2 (Thr313) | Akt2 (Tyr316) |
| Akt2 (Tyr475) | Akt3 (Ser472) | Akt3 (Ser474) | Akt3 (Ser476) |
| Akt3 (Thr298) | Akt3 (Thr305) | Akt3 (Tyr473) | ATR (Ser435) |
| ATR (Ser436) | BAD (Ser71) | BAD (Ser74) | BAD (Ser75) |
| BAD (Ser91) | BAD (Ser97) | BAD (Ser99) | BAD (Thr80) |
| BAD (Tyr76) | CTNND1 iso3 (Ser168) | CTNND1 iso3 (Thr147) | CTNND1 iso3 (Thr149) |
| CTNND1 iso3 (Thr152) | CTNND1 iso4 (Ser168) | CTNND1 iso4 (Thr147) | CTNND1 iso4 (Thr149) |
| CTNND1 iso4 (Thr152) | CTNND1 (Ser168) | CTNND1 (Thr147) | CTNND1 (Thr149) |
| CTNND1 (Thr152) | DNMT1 iso2 (Ser748) | DNMT1 (Ser732) | Dok1 (Tyr341) |
| Dok2 (Tyr299) | eIF4H (Ser230) | eIF4H (Thr217) | eIF4H (Thr220) |
| EphA2 (Thr593) | EphA2 (Thr771) | EphA2 (Thr773) | EphA2 (Thr774) |
| EphA2 (Tyr588) | EphA2 (Tyr772) | FAK iso2 (Ser211) | FAK iso2 (Ser422) |
| FAK iso2 (Thr227) | FAK iso2 (Tyr216) | FAK iso2 (Tyr424) | FAK iso2 (Tyr425) |
| FAK (Ser392) | FAK (Ser574) | FAK (Thr408) | FAK (Tyr397) |
| FAK (Tyr576) | FAK (Tyr577) | FOXK1 (Thr273) | FOXO1A (Ser256) |
| FOXO3A (Ser43) | FOXO3A (Ser48) | FOXO3A (Ser55) | FOXO3A (Thr51) |
| Fyn iso2 (Tyr417) | Fyn (Tyr420) | GAPDH (Thr153) | GOLGA3 (Ser1430) |
| GOLGA3 (Ser983) | GOLGA4 (Ser40) | GOLGA4 (Ser41) | GOLGA4 (Thr39) |
| GOLGA4 (Thr43) | GSK3A (Ser20) | GSK3A (Ser21) | GSK3A (Ser39) |
| GSK3A (Ser41) | GSK3A (Thr19) | GSK3A (Thr46) | HGK iso5 (Ser908) |
| HGK iso5 (Thr187) | HGK iso5 (Thr910) | HGK iso7 (Ser463) | HGK iso7 (Ser934) |
| HGK iso7 (Thr127) | HGK iso7 (Thr936) | HGK (Ser523) | HGK (Ser913) |
| HGK (Thr187) | HGK (Thr915) | HRas (Tyr32) | HRas (Tyr64) |
| IRS1 (Ser303) | IRS1 (Ser307) | IRS1 (Ser312) | IRS1 (Ser315) |
| IRS1 (Ser323) | IRS1 (Ser603) | IRS1 (Ser604) | IRS1 (Ser616) |
| IRS1 (Thr305) | IRS1 (Thr309) | IRS1 (Thr311) | IRS1 (Thr608) |
| IRS1 (Tyr1179) | IRS1 (Tyr612) | IRS2 (Ser594) | IRS2 (Tyr598) |
| KHSRP (Ser132) | KRas iso2 (Tyr32) | KRas iso2 (Tyr64) | KRas (Tyr32) |
| KRas (Tyr64) | lamin A/C iso2 (Ser404) | lamin A/C iso2 (Ser406) | lamin A/C (Ser404) |
| lamin A/C (Ser406) | Lck (Tyr394) | LOC647000 (Ser41) | MEK1 (Ser218) |
| MEK1 (Ser222) | MEK1 (Thr226) | MEK2 (Ser222) | MEK2 (Ser226) |
| MEK2 (Thr230) | MINK iso4 (Thr187) | MINK (Thr187) | MST3 iso2 (Thr170) |
| MST3 iso2 (Thr172) | MST3 iso2 (Thr178) | MST3 iso2 (Thr182) | MST3 (Thr182) |
| MST3 (Thr184) | MST3 (Thr190) | MST3 (Thr194) | MST4 (Thr320) |
| MST4 (Thr327) | mTOR (Ser2442) | mTOR (Ser2448) | mTOR (Ser2450) |
| mTOR (Ser2454) | mTOR (Ser2478) | mTOR (Ser2481) | mTOR (Thr2444) |
| mTOR (Thr2446) | mTOR (Thr2471) | mTOR (Thr2473) | mTOR (Thr2474) |
| mTOR (Tyr2449) | MYPT1 (Ser802) | MYPT1 (Ser910) | MYPT1 (Thr453) |
| MYPT1 (Tyr446) | MYPT1 (Tyr766) | MYPT1 (Tyr901) | MYPT1 (Tyr911) |
| NDRG1 (Ser344) | NDRG1 (Ser352) | NDRG1 (Ser354) | NDRG1 (Ser357) |
| NDRG1 (Ser362) | NDRG1 (Ser364) | NDRG1 (Ser367) | NDRG1 (Ser378) |
| NDRG1 (Ser384) | NDRG1 (Ser389) | NDRG1 (Ser393) | NDRG1 (Thr346) |
| NDRG1 (Thr356) | NDRG1 (Thr360) | NDRG1 (Thr366) | NDRG1 (Thr375) |
| NF-GMB iso2 (Ser134) | NF-GMB iso2 (Tyr131) | NF-GMB (Ser134) | NF-GMB (Tyr131) |
| NF-κB p105 (Ser907) | NRas (Tyr32) | NRas (Tyr64) | Nur77 (Ser351) |
| Nur77 (Ser360) | PAK2 (Ser19) | PAK2 (Ser20) | PAK2 (Ser32) |
| PAK2 (Ser36) | PAK2 (Ser42) | PAK2 (Thr21) | PAK2 (Thr25) |
| PAR-4 (Ser231) | PAR-4 (Ser233) | PAR-4 (Thr229) | PAR-4 (Tyr177) |
| PDK1 iso2 (Ser114) | PDK1 (Ser241) | PDPK2 (Ser214) | PIK3CA (Ser507) |
| PIK3CA (Ser509) | PIK3R1 (Tyr467) | PIK3R2 (Tyr464) | PIK3R3 (Tyr199) |
| PIK3R5 (Ser507) | PIK4CB iso2 (Ser256) | PIK4CB (Ser256) | PKA RI-α (Ser375) |
| PKCα (Thr494) | PKCα (Thr497) | PKCα (Tyr504) | PKCβ iso2 (Thr498) |
| PKCβ iso2 (Thr500) | PKCβ (Thr498) | PKCβ (Thr500) | PKCI (Thr412) |
| PKD2 (Ser375) | PRAS40 (Ser203) | PRAS40 (Thr266) | PTEN (Ser385) |
| RANBP3 iso3 (Ser57) | RANBP3 iso3 (Ser58) | RANBP3 iso3 (Thr56) | RANBP3 iso3 (Thr60) |
| RANBP3 (Ser125) | RANBP3 (Ser126) | RANBP3 (Thr124) | RANBP3 (Thr128) |
| Raptor (Ser719) | Raptor (Ser722) | Rictor (Ser1320) | RSK2 (Ser386) |
| S6 (Ser235) | S6 (Ser236) | S6 (Ser240) | S6 (Ser242) |
| S6 (Ser244) | S6 (Ser246) | S6 (Ser247) | S6 (Thr241) |
| SGK3 (Ser315) | SGK3 (Thr318) | Shb (Ser323) | Shb (Tyr336) |
| SHIP-2 (Tyr886) | Src (Tyr419) | TUBA1A (Ser287) | TUBA1B (Ser287) |
| TUBA3C (Ser287) | TUBA3D (Ser287) | TUBA3E (Ser287) | TUBA4A (Ser287) |
| TUBB2A (Ser339) | TUBB2B (Ser339) | TUBB2C (Ser339) | TUBB3 (Ser339) |
| TUBB4 (Ser339) | TUBB6 (Ser339) | TUBB (Ser339) | XIAP (Ser123) |
| YB-1 (Ser102) | YB-1 (Thr108) | YB-1 (Tyr99) | Yes (Tyr426) |
| YSK1 (Thr166) | YSK1 (Thr168) | YSK1 (Thr174) | YSK1 (Thr178) |
