PTMScan^® Pathway Based Enrichment Kits

Bridging Discovery and Targeted Approaches

Perform mass spectroscopy-based screening and quantification of defined sets of thousands of protein-sites in important signaling pathways with PTMScan^® pathway based enrichment kits. These kits include the same antibodies and technology used in the PTMScan^® Direct Services, employing multiplexed beads labeled with standard site-specific antibodies for defined targets within known pathways of interest.

Coverage includes both total and phosphorylated protein sites at key signaling nodes critical for drug discovery, biomarker discovery, and diagnostic development. This approach offers comprehensive analysis of signaling with reduced likelihood of missing important data points, with the flexibility of customizable, targeted follow-up experiments.

Multi-Pathway Enrichment kit

Relative category distribution of proteins from peptides identified from LC-MS/MS experiments of human cancer cell lines using the PTMScan^® Multi-Pathway Enrichment kit.

Pathway Coverage

The PTMScan^® Multi-Pathway Enrichment kit provides a robust method to identify and quantitatively profile >4000 unique sites and >800 key proteins with known functions in a single experiment.

Download a list of protein-sites covered by the Multi-Pathway Enrichment Kit.

Highlighted purple nodes designate targeted protein modification sites within signaling pathways that can be monitored with the PTMScan^® Multi-Pathway Enrichment kit.

Monitored phosphorylation in Adherens Junction Dynamics

Monitored phosphorylation in AMPK Signaling

Monitored phosphorylation in B Cell Receptor Signaling

Monitored phosphorylation in Cell Cycle Control: G1/S Checkpoint

Monitored phosphorylation in Cell Cycle Control: G2/M DNA Damage Checkpoint

Monitored phosphorylation in ErbB / HER Signaling

Monitored phosphorylation in G Protein-coupled Receptors Signaling to MAPK / Erk

Monitored phosphorylation in Insulin Receptor Signaling

Monitored phosphorylation in Jak / Stat Signaling: IL-6 Receptor Family

Monitored phosphorylation in Mitogen Activated Protein Kinase Signaling Cascades

Monitored phosphorylation in MAPK / Erk in Growth and Differentiation

Monitored phosphorylation in NF-κB Signaling

Monitored phosphorylation in PI3 Kinase / Akt Signaling

Monitored phosphorylation in Regulation of Actin Dynamics

Monitored phosphorylation in Regulation of Microtubule Dynamics

Monitored phosphorylation in SAPK / JNK Signaling Cascades

Monitored phosphorylation in Signaling Pathways Activating p38 MAPK

Monitored phosphorylation in T Cell Receptor Signaling

Monitored phosphorylation in Toll-like Receptor Signaling

Select a Pathway Adherens Junction Dynamics AMPK Signaling B Cell Receptor Signaling Cell Cycle Control: G1/S Checkpoint Cell Cycle Control: G2/M DNA Damage Checkpoint ErbB / HER Signaling G Protein-coupled Receptors Signaling to MAPK / Erk Insulin Receptor Signaling Jak / Stat Signaling: IL-6 Receptor Family Mitogen Activated Protein Kinase Signaling Cascades MAPK / Erk in Growth and Differentiation NF-κB Signaling PI3 Kinase / Akt Signaling Regulation of Actin Dynamics Regulation of Microtubule Dynamics SAPK / JNK Signaling Cascades Signaling Pathways Activating p38 MAPK T Cell Receptor Signaling Toll-like Receptor Signaling

Hierarchical Clustering of Results

Hierarchical clustering of enriched peptides by relative abundance using the PTMScan^® Multi-Pathway Enrichment Kit. Left: each row represents the results of a unique peptide against the tissue type. Right: detailed view of Akt peptides identified and quantified. Data was calculated on a 0 (white, lowest abundance) to 1 (blue, highest abundance) scale.

Bridge The Gap

The PTMScan^® Multi-Pathway Enrichment kit offers the flexibility to assay signaling pathway activities across cell lines or experimental treatments with customizable follow-up experiments. For example, a small number of samples may be run in order to narrow down to the most abundant or relevant targets. The same kit can be subsequently used with a larger number of samples, focusing on the priority targets established in the first step. Validation of PTMScan^® results can be performed using AQUA synthetic peptides or other peptide standards.

All PTMScan^® kits come with reagents for 10 assays and detailed protocols. Purchase of the kit also includes a Limited Use License to perform PTMScan^® Technology.